منابع مشابه
Cation Transport in Escherichia coli
K influx and net K flux have been measured in suspensions of chloramphenicol-arrested Escherichia coli. The rate of K exchange in the steady state was independent of the K concentration of the medium over a 200-fold range. Under a number of experimental conditions the rate of exchange may be considerably increased or decreased without changing the cellular K content. These results show that und...
متن کاملCation Transport in Escherichia coli
The present study is concerned with the measurement of the unidirectional K flux in E, coli. Methods are described by means of which a fairly dense suspension of cells may be maintained in a well defined steady-state with respect to the intracellular K concentration and the pH of the medium. The kinetics of K 4~ exchange under these conditions are consistent with the presence of a single intrac...
متن کاملCation transport in Escherichia coli. VIII. Potassium transport mutants
Analysis of K transport mutants indicates the existence of four separate K uptake systems in Escherichia coli K-12. A high affinity system called Kdp has a Km of 2 muM, and Vmax at 37 degrees C of 150 mumol/g min. This system is repressed by growth in high concentrations of K. Two constitutive systems, TrkA and TrkD, have Km's of 1.5 and 0.5 mM and Vmax's of 550 and 40 at 37 and 30 degrees C, r...
متن کاملCation Transport in Escherichia coli VIII. Potassium Transport Mutants
Analysis of K transport mutants indicates the existence of four separate K uptake systems in Escherichia coli K-12. A high affinity system called Kdp has a Km of 2 p.M, and Vmax at 37°C of 150/zmol/g min. This system is repressed by growth in high concentrations of K. Two constitutive systems, TrkA and TrkD, have K,,'s of 1.5 and 0.5 mM and Vm~x's of 550 and 40 at 37 and 30°C, respectively. Mut...
متن کاملCation transport in Escherichia coli. IX. Regulation of K transport
Kinetics of K exchange in the steady state and of net K uptake after osmotic upshock are reported for the four K transport systems of Escherichia coli: Kdp, TrkA, TrkD, and TrkF. Energy requirements for K exchange are reported for the Kdp and TrkA systems. For each system, kinetics of these two modes of K transport differ from those for net K uptake by K-depleted cells (Rhoads, D. B. F.B. Walt...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1966
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.49.3.469